정양삼한의원

논문 정양삼

> paper > 정양삼

고지방식이 수컷 마우스 비만모델에서 micro-CT를 이용한 麻黃과 魔芋의 복부비만 조절효과

Introduction
고지방식이 수컷 마우스 비만모델에서 micro-CT를 이용한 麻黃과 魔芋의 복부비만 조절효과

원찬욱¹, 정양삼¹, 윤기현¹, 이희영¹, 윤미정², 김보경³, 박선동⁴, 신순식¹
동의대학교 한의과대학 ¹방제학교실, ²신경정신과학교실 및 한의학연구소,
³목원대학교 바이오건강학부, ⁴동국대학교 한의과대학 방제학교실

ABSTRACT

Herba Ephedrae and Rhizoma Amorphophalli modulates visceral obesity in micro-CT of high fat induced obese male mice


Chan-Uk Won¹, Yang-Sam Jung¹, Ki-Hyeon Yoon¹, Hee-Young Lee¹,
Mi-Chung Yoon², Bo-Kyung Kim ³, Sun-Dong Park⁴, Soon-Shik Shin¹
¹Department of Formula Sciences and ³Department of Neuropsychiatry, College of Oriental Medicine & Research Institute of Oriental Medicine, Dong-Eui University
²Department of Life Sciences, Mokwon University
⁴Department of Formula Sciences, College of Oriental Medicine, Dongguk University

Objectives
: We investigated the effects of Herba Ephedrae and Rhizoma Amorphophalli on high fat diet induced obese male mice.

Methods : 8 weeks old, high fat diet induced obese male mice were divided into 5 groups: C57BL/6 normal control, obese vehicle control, GGEx55 (Herba Ephedrae), GGEx61 (Rhizoma Amorphophalli), GGEx62 (Herba Ephedrae + Rhizoma Amorphophalli). After mice were treated with GGEx for 8 weeks, we measured body weight gain, food intake, feeding efficiency ratio, rectal temperature, fat weight, plasma leptin and lipid levels. We also took micro-computerized axial tomography (micro-CT) on the mice.

Results :
1. GGEx55 and GGEx62 groups significantly decreased body weight gain and feeding efficiency ratio compared with vehicle control. But they significantly increased rectal temperature.
2. Plasma total cholesterol and LDL-cholesterol concentrations were significantly increased by GGEx55 groups, whereas were significantly decreased by GGEx62 groups compared with vehicle control.
3. GGEx55 and GGEx62 groups significantly decreased total, subcutaneous and visceral fat as well as fat areas in micro-CT analysis of abdomen compared with vehicle control.
4. Plasma GOT and GPT concentrations were significantly increased by GGEx55 groups compared with vehicle control.

Conclusions : These results demonstrate that GGEx55 and GGEx62 effectively reduces body weight gain, feeding efficiency ratio in high fat diet induced obese mice, leading to the modulation of obesity. In addition, GGEx55 and GGEx62 decreases visceral adipose tissue mass and improves plasma lipids, suggesting that GGEx55 and GGEx62 may act as a therapeutic agent for obesity.
Key word : Herba Ephedrae, Rhizoma Amorphophalli, high fat diet induced obese male mouse, obesity